In a key paper, Stoeckenius (1962) provided clear pictures of the three-layer structure. He then described in both words and diagrams how the lipid bilayer results in a three-layer image.

It’s made of three layers, including an outer lipid layer. The lipid layer works to stabilize the tear film. This stops tears from evaporating, which keeps the eye surface smooth and lubricated.

A new tissue expansion method developed by researchers at HHMI's Janelia Research Campus and the University of ...