The Klenow fragment of DNA polymerase-I was the only enzyme available, 7dG and dITP were almost unheard of, and the equipment was often mismatched and kludgy. In-house (or even, at times, in-lab) ...

4. To the reaction mix from Step 3, add 4 μl of deoxynucleoside triphosphate (dNTP) mixture (20 mM) and 1 μl (10 units) of the Klenow fragment of Escherichia coli DNA polymerase I. Incubate the ...

This study provides a new perspective on Japan's stagnant aggregate productivity by extending the Hsieh and Klenow (2009) framework to account for productivity growth, entry and exit, and product ...