Interferon regulatory factor 8 (IRF8) is well known for its roles in immune cell differentiation and tumor pathogenesis; however, its role in regulating glucose homeostasis and lipid metabolism ...

(J, K) Protein levels (J) and relative mRNA expression (K) of Pparγ and its target genes were determined in MPHs transfected with si-NC + Vector, si-IRF8 + Vector, si-NC + BMAL1 OE, and si-IRF8 ...

MPHs were transfected with Ad-GFP or Ad-IRF8 to modulate IRF8 expression and were treated with GW9662 (10 μM) to inhibit PPARγ, followed by treatment with 0.1 mM palmitic acid (PA) for 24 h.

The Irf8 floxed targeted mutated mouse strain can be used to selectively ablate expression of IRF8 in any cell type in which a Cre recombinase gene is activated.