The method described here 1 uses carrier ampholytes to form a pH gradient in a long, thin (1.2-mm) focusing gel composed of a low percentage (2.7%) of acrylamide and containing 9.5 M urea and 2% ...

1 | Cast a 1% agarose gel in 1× TAFE gel buffer (20 mM Tris-acetate (pH 8.2), 0.5 mM EDTA) without ethidium bromide and allow the gel to set. Run the gel in the same buffer solution as used for ...