In 1955, Oliver Smithies published a groundbreaking Biochemical Journal article, introducing the world to gel electrophoresis ... precise column-based strategies—for analysis of protein mixtures.

After concentrating to 10 mM, protein was applied to a Superdex 75 gel filtration column (Pharmacia) for final purification and characterization. Crystallization and data collection. Hanging drops ...

If the protein has been purified to homogeneity ... For example, if you only have a 2 mL fraction of the gel filtration column, you would probably get the highest purity of phosphatase; however, this ...