Mutagenesis of amino acids bound to the fluorophore was key to changing the excitation–emission spectrum of GFP, and it produced GFP variants with different colours and capacities for changing ...

Although 3 of 11 GFP mutants were phosphorylated by PKA ... The ART fluorescence emission spectra were measured in vitro with a spectrofluorometer FP-750 (JASCO, Tokyo, Japan) before and after ...