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Engineers and biologists at Dartmouth College have found that, just like word order affects meaning in a sentence, the ...
Gene syntax also affects the behavior of genetic circuits, and contributes to unpredictable outcomes in genetic networks.
Plasmid vectors pET11a-link-NGFP and pMRBAD-link-CGFP and positive control vectors pET11a-Z-NGFP and pMRBAD-Z-CGFP are available upon request (Fig. 1) Figure 1: GFP-fragment reassembly vectors and ...
As a next step, the investigators replaced c-myc with the considerably larger coding region for enhanced green fluorescent protein (EGFP). Transfection of this modified HIV plasmid into HeLa cells ...
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