In the first step, the 2'-OH of an adenosine in the intron (the branch site A) attacks the phosphodiester bond at the 5' splice site to release the upstream exon and form a branched or lariat ...

This spliceosome is made up of variety of protein and RNA components, and for each splicing process, it is reassembled at the sites where an exon joins an intron and the intron in turn connects to ...

Computational prediction of gene structure is crucial for interpreting genomic sequences. But how do the algorithms involved work and how accurate are they? Expression-based systems that use only ...