The transcription of eGFP is driven by the IRES so the eGFP expression will be slightly less than seen in the Ad5-CMV-eGFP vector. Ad5-CMV-Empty Ad5-Empty virus contains a CMV promoter and poly(A) but ...

and EGFP and EGFP alone were from Clontech (Palo Alto, CA). The nonGFP-linked pMV7-PKC-β(I) expression vector used in control studies was from I. B. Weinstein (New York, NY). Cell transfection ...

Samples of each sorted phenotype were infected immediately after FACS with supernatant derived from the NIT-EGFP retrovirus, a vesicular stomatitis virus G (VSV-G) pseudotyped amphotropic vector ...