As a next step, the investigators replaced c-myc with the considerably larger coding region for enhanced green fluorescent protein (EGFP). Transfection of this modified HIV plasmid into HeLa cells ...

Both P/musashi1:hGFP- and E/nestin:EGFP-sorted cells were multipotent: limiting dilution with clonal expansion as neurospheres, in tandem with retroviral lineage analysis and xenograft to E17 and ...