Examining cells for eGFP expression following transduction with Ad5-CMV-eGFP using a fluorescent microscope is a quick method for estimating transduction efficiency. Ad5-CMV-Cre-eGFP expresses the Cre ...

Figure 1D. In the absent of Cre, EGFP was expressed in the DA (arrowheads), and no red fluorescent signal was not detected in the TagRFP channel. Scale bars, 100 μm.