qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...

Researchers have developed a personalized blood test that may offer a faster, less invasive way to track high-grade glioma progression.

Dubbed quantitative real-time PCR (qPCR), this technique makes it possible to measure the amount of DNA produced during each PCR cycle. This refinement involves the use of fluorescent dyes or ...

Among the methods of detecting residual DNA, qPCR is the most widely used for residual DNA quantitation due to its sensitivity, accuracy, precision, and time-saving capability. In this webinar, we ...

Researchers have developed a light-induced DNA detection method that enables rapid, PCR-free genetic analysis. Their technique offers ultra-sensitive mutation detection in just five minutes, reducing ...

Initially, ethidium bromide (EtBr) was commonly used as a DNA-binding fluorescent dye for monitoring qPCR. Its drawbacks, including being a potential human mutagen, led to the introduction of SYBR® ...

The GoTaq ® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing ...

“The most reliable method to detect a disease—because it looks at areas of the genome unique to that virus—is a PCR method called quantitative PCR (qPCR),” Paliulis says. Also based on DNA ...

One assay applies to bioprocesses that use the HEK293 human embryonic kidney cell line, often used in gene therapy development.

BHQnova ™ Probes are double-quenched hydrolysis probes with enhanced quenching and greater signal-to-noise ratio for long probes (>25 bases). Such probes consist of a 5' fluorophore dye, a 3 ...

The research-use-only assay, which includes a qPCR detection kit and an isolation kit for cell-free DNA and exosomal RNA, will also be featured at the ongoing San Antonio Breast Cancer Symposium.

In January 2022, the NC State team attempted to amplify Bartonella and Borrelia DNA from patient blood samples via qPCR and digital droplet PCR testing, but results were negative. In June 2022 ...