Thermo Fisher researchers took a standard human carcinoma line (A-431) and used a western blot to get a baseline for the binding signal. They then used CRISPR-Cas9 to eliminate the target gene and ...

In a new study published in Nature titled, “Custom CRISPR-Cas9 PAM variants via scalable engineering and machine learning,” researchers from Massachusetts General Hospital (MGH) and Harvard ...

We employed the CRISPR/Cas9 genome editing platform for the generation ... fusion protein to be an 86 kDa fragment as expected by western blotting (data not shown). Functional testing of the ...

In December 2020, the New England Journal of Medicine published a paper titled CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia, marking an exciting new chapter in the treatment of ...