Genome editing has advanced at a rapid pace with promising results for treating genetic conditions—but there is always room ...

Researchers have developed a machine learning model that predicts Cas9 proteins that can be tailored with designer properties for therapeutic use.

with a transcription activation domain (VPR). When exposed to blue light, this transcription factor induces guide RNA (gRNA) in cells, which then binds to Cas9, leading to the editing of selected ...

The version of Cas9 used in prime editing has one of its two nuclease domains deactivated, turning it into a “nickase” that will only cut one of the DNA strands, rather than both. So, once the pegRNA ...

CRISPR-Cas9 is not the first method available to scientists for modifying ... Several zinc ions are bound to the amino acid chain in the DNA-binding domain. This forms loops as a result (‘zinc fingers ...

looking for any that shared a similar domain. To find more distantly related proteins, they used an iterative process: from ...

Deanna earned their PhD in cellular biology from McGill University in 2020 and has a professional background in medical writing. They are an associate science editor at The Scientist. View Full ...

The Cas9 protein is the most widely used by scientists. This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it ...

In December 2020, the New England Journal of Medicine published a paper titled CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia, marking an exciting new chapter in the treatment of ...