Gene-editing tools such as CRISPR/Cas9 can be used to create isogenic cell lines, which can be further used to model a specific patient population. An isogenic cell line was created to model ...

Ryotaro Hashizume and colleagues used the CRISPR-Cas9 gene editing system to cleave the third chromosome in previously ...

We provide transgene expression in mammalian cell lines using lentiviral and retroviral transduction and gene knockout and knockin using CRISPR/Cas9 technologies. 1. STABLE CELL LINE GENERATION ...

SINGLE GUIDE RNA DESIGN AND PCR OPTIMIZATION We design and optimize CRISPR/Cas9 single or double guide RNAs for genome editing projects in mammalian cell lines. The service can be requested together ...

To characterize this pathway, the researchers used CRISPR-Cas9 screens on two metastatic prostate cancer cell lines to identify key genes. One standout was a gene that encodes vacuolar protein sorting ...

A study of human tumor cells implanted in mice from the MacPherson Lab at Fred Hutch describes a new way to screen for the ...

tested in human and mouse cells, rewrites DNA by only cutting a single strand to add, remove, or replace base pairs. The method may allow researchers to edit more types of genetic mutations than ...

Scientists have bred spiders that weave red draglines, proving that the animals’ silk can be genetically re-engineered.

Researchers in Korea generated a new method for generating CHO cell lines with significantly higher productivity, efficiency, ...

In December 2020, the New England Journal of Medicine published a paper titled CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia, marking an exciting new chapter in the treatment of ...

Targeting the intracellular immune checkpoint CISH with CRISPR-Cas9-edited tumor-infiltrating lymphocytes (TILs) may be ...