For each of two nucleases—Cas9 from Streptococcus pyogenes and Cas12 from Staphylococcus aureus—they identified three short sequences, about eight amino acids long, that evoked an immune response.

Class II Cas9 and Class V Cas12 are currently widely utilized as gene-editing tools. However, both the Cas9 and Cas12 ...

Deanna earned their PhD in cellular biology from McGill University in 2020 and has a professional background in medical writing. They are an associate science editor at The Scientist. View Full ...

In a new study published in Nature titled, “Custom CRISPR-Cas9 PAM variants via scalable engineering and machine learning,” researchers from Massachusetts General Hospital (MGH) and Harvard ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

The Cas9 protein is the most widely used by scientists. This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it ...

In December 2020, the New England Journal of Medicine published a paper titled CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia, marking an exciting new chapter in the treatment of ...