3) thus preventing discrimination of CFP and photobleached YFP on the basis of their emission or excitation spectra. During photobleaching FRET experiments, any increase in the CFP channel could ...

Figure 1: ATP concentration affects signal efficacy of CFP-YFP–based FRET sensors. It is important to note further that the ratio changes (8–45% change in YFP/CFP ratio) are within the range ...