Shown above are primary dermal fibroblasts transfected with EGFP-C1 plasmids. Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert!

In addition, the inventors inserted an eGFP gene into the first gene position (promoter proximal). The resulting virus is replication-competent and efficiently expresses GFP in infected cells.

We next aimed to verify the dCas9-EGFP signals with FISH using probes targeting repetitive sequences so that a single FISH probe could access many sites. We used another two sgRNA sequences ...