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Figure 1. Isolated Glomerular ultrafiltration coefficient (LpA) assay. Following isolation, glomeruli are incubated at 37°C in 1% BSA solution (with or without test solutions). Glomeruli are then ...
The PamChips® are blocked in 2% Bovine Serum Albumin (BSA). Following protein concentration determination (by BCA assay), 5-10 μg of protein for the PTK chip (~1 μg for the STK) will be loaded per ...