E/nestin:EGFP and P/musashi1:hGFP-sorted cells were ... their brains were then cut and immunostained for anti-human nuclear antigen together with neuronal and glial phenotypic markers, in order ...

In addition, the inventors inserted an eGFP gene into the first gene position (promoter proximal). The resulting virus is replication-competent and efficiently expresses GFP in infected cells.