Probes then specifically bind to AAV capsids. Oligos on these probes ligate together when in close proximity. ddPCR technology then amplifies and detects the ligated oligo pairs, while an ITR-2 ...

In 2014, researchers combined DNA barcoding with next-generation sequencing (NGS) to develop the adeno-associated virus (AAV) barcode-seq technology for streamlining AAV screening. Using this method, ...

Previous efforts to circumvent the problem of the size limitation imposed by the AAV vector have focused mainly on altering the candidate genes into ‘mini-expression cassettes’ suitable for ...