Genome editing via the all-in-one AAV vector showed considerable efficiency and specificity. It showed on-target T to C conversion in human cells, expressing the GJB2 R75W mutation, repaired the ...

In 2014, researchers combined DNA barcoding with next-generation sequencing (NGS) to develop the adeno-associated virus (AAV) barcode-seq technology for streamlining AAV screening. Using this method, ...

6 To generate a library of new capsids, Deverman edited a section of the AAV genome that contained a stretch of seven amino acids that he found can tolerate a bit of change but still form a capsid. By ...

ddPCR technology then amplifies and detects the ligated oligo pairs, while an ITR-2 assay simultaneously detects the AAV genome. Software analyzes the signals from each capsid type to calculate ...

Gator Bio is the market’s sole solutions supplier that provides assays for genome titer and integrity, capsid titer, and empty-full ratio on a single automated platform. The AAV genome titer and ...

This in silico approach aims to increase the fitness of clinical adeno-associated virus (AAV) capsids to make gene therapies more economically viable for patients. Developing AAV capsids with ...

Gene therapy represents one of the fastest growing biotherapeutics worldwide, with AAV at the forefront. This rapid growth is ...

This system also confers additional stability on the baculovirus genome, and gives rise to AAV vectors that achieve higher levels of infectivity — potentially enabling greater therapeutic ...

To overcome these barriers, researchers explored lentiviral vectors, which integrate into the genome and bypass AAV-specific limitations. In the study, "Lentiviral Gene Therapy with CD34 ...